Discovery

Caused by the human immunodeficiency virus (HIV), AIDS leaves an infected person vulnerable to opportunistic infections. Since HIV was first identified as the cause of AIDS in 1983, a variety of tests have been developed that help diagnose HIV infection.

Doctors determine if HIV is present in the body by identifying HIV antibodies. The presence of the antibodies indicates HIV infection because these antibodies form in the body only when HIV is present.

The standard test to detect HIV antibodies in the blood is the enzyme-linked immunosorbent assay (ELISA). In this test, a blood sample is mixed with proteins from HIV. If the blood contains HIV antibodies, they attach to the HIV proteins, producing a telltale color change in the mixture.

Doctors routinely confirm a positive result from an ELISA test by using the Western Blot test, which can detect lower levels of HIV antibodies. In this test a blood sample is applied to a paper strip containing HIV proteins. If HIV antibodies are present in the blood, they bind to the HIV proteins, producing a color change on the paper. The combination of the ELISA and the Western Blot test is more than 99.9 percent accurate in detecting HIV infection within 12 weeks following exposure.

Once tests confirm an HIV infection, doctors monitor the health of the infected person’s immune system by periodically measuring CD4 cell counts in the blood. The progressive loss of CD4 cells corresponds to a worsening of the disease as the immune system becomes increasingly impaired.

Doctors also measure the viral load—the amount of the virus in the blood—using polymerase chain reaction (PCR) technology. PCR tests measure the level of viral ribonucleic acid (RNA), a type of nucleic acid, in blood to determine the rate of HIV growth in an infected person. Knowing the viral load helps doctors estimate an infected person’s survival time. For example, studies show that without treatment, the average survival time for people with an HIV viral load greater than 30,000 per microliter of blood is 4.4 years, while those with a viral load below 10,000 per microliter of blood live for an average of ten years.

Article Taken From : Encarta

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